murine hepatocyte cell line fl83b  (BioResource International Inc)

Journal:

Article Title: Type IV Pili in Francisella tularensis : Roles of pilF and pilT in Fiber Assembly, Host Cell Adherence, and Virulence ▿

doi: 10.1128/IAI.01726-07

Figure Lengend Snippet: pilF and pilT contribute to host cell adherence. Cells were treated with cytochalasin D to inhibit the internalization of bacteria and then infected with wild-type LVS (WT), DTLB (ΔpilF), DTLB complemented with pilF (ΔpilF/pGPB), or the pilT::HimarFT mutant. The graphs show the CFU of bacteria that adhered to murine bone marrow-derived macrophages (A), A549 human lung epithelial cells (B), and FL83B murine hepatocytes (C). Error bars indicate the standard deviation of triplicate samples. P values were calculated by using a paired t test, comparing DTLB or pilT:HimarFT with the wild-type LVS or comparing DTLB/pGPB with DTLB (*, P < 0.05; **, P < 0.02).

Article Snippet: The murine hepatocyte cell line FL83B, derived from the normal liver of a 15- to 17-day-old fetal mouse, was purchased from the ATCC.

Techniques: Bacteria, Infection, Mutagenesis, Derivative Assay, Standard Deviation

Journal: Molecular therapy : the journal of the American Society of Gene Therapy

Article Title: In vivo hepatic adenoviral gene delivery occurs independently of the coxsackievirus-adenovirus receptor.

doi: 10.1006/mthe.2002.0613

Figure Lengend Snippet: FIG. 7. In vitro adenoviral gene delivery to human and mouse hepatocytes. Human hepatocarcinoma cells, HepG2 (A), the mouse hepatocyte cell line, FL83B (B), or primary CD-1 mouse hepatocytes (C) were infected with each adenoviral vector from 0 up to 12,500 particles per cell (PPC) of Av3nBg (open circle) or Av3nBgFKO1 (filled circle) for 1 hour at 37C. After 24 hours the cells were fixed and stained with X-gal. The percentage of transduced cells was determined per high-power field for each vector dose. The data represent the mean percentage of transduction ± SD (n = 3 wells) from one representative experiment.

Article Snippet: HeLa (human cervical carcinoma; ATCC CCL2), HepG2 (human hepatocarcinoma; ATCC HB-8065), and FL83B, a fetal murine hepatocyte cell line (ATCC CRL-2390), were obtained from the American Type Culture Collection (Manassas, VA), and we cultured each cell line in the recommended medium.

Techniques: In Vitro, Infection, Plasmid Preparation, Staining, Transduction

Journal: Molecular therapy : the journal of the American Society of Gene Therapy

Article Title: In vivo hepatic adenoviral gene delivery occurs independently of the coxsackievirus-adenovirus receptor.

doi: 10.1006/mthe.2002.0613

Figure Lengend Snippet: FIG. 8. Competition analysis of adenoviral transduction of primary murine hepatocytes. CD-1 murine primary hepatocytes were transduced with Av3nBg (wt) at 2500 PPC or Av3nBgFKO1 (KO1) at 12,500 PPC in the presence or absence of fiber knob (10 g/ml), an RGD peptide (200 g/ml), or heparin (3 mg/ml) for 1 hour at 37C. After 24 hours, the cells were fixed and stained with X-gal. The number of transduced cells was determined per high-power field for each condition and was expressed as a percentage of the total trans- duction in the absence of competitor. The level of transduction for each vec- tor in the absence of competitor was defined as 100%. The data show the aver- age percentage control ± SD from a representative experiment.

Article Snippet: HeLa (human cervical carcinoma; ATCC CCL2), HepG2 (human hepatocarcinoma; ATCC HB-8065), and FL83B, a fetal murine hepatocyte cell line (ATCC CRL-2390), were obtained from the American Type Culture Collection (Manassas, VA), and we cultured each cell line in the recommended medium.

Techniques: Transduction, Staining, Control